Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

Download scientific diagram | Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and CLIP-tag fused to the protein of interest bind benzylguanine-or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/ labeling steps can be added.   from publication: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags | Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to | Protein Tagging, Electron Microscopy and Correlative Light Electron Microscopy | ResearchGate, the professional network for scientists.

Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags

Bloomington Drosophila Stock Center: Indiana University Bloomington

Multivalent Chelators for In Vivo Protein Labeling - Wieneke - 2019 - Angewandte Chemie International Edition - Wiley Online Library

SNARE Complex Formation Is Triggered by Ca2+ and Drives Membrane Fusion: Cell

Jean-Marc VERBAVATZ, Group Leader, Institut Jacques Monod, Paris, IJM

The Evolution of SNAP-Tag Labels

Protein Labeling

Evaluation of Fluorophores to Label SNAP-Tag Fused Proteins for Multicolor Single-Molecule Tracking Microscopy in Live Cells: Biophysical Journal

IJMS, Free Full-Text

Illustration of methods used for fluorescence labelling. (A)