Site-specific protein immobilization by fusion to self-labeling tags.

Download scientific diagram | Site-specific protein immobilization by fusion to self-labeling tags. (a) The SNAP-tag, 20 kDa in size, reacts with O 6 -benzylguanine. (b) The CLIP-tag (also 20 kDa) reacts with O 2 -benzylcytosine substrates, whereas the HaloTag (33 kDa) forms a covalent bond through nucleophilic displacement of halides from alkyl halide substrates.   from publication: Recent advances in covalent, site-specific protein immobilization | The properties of biosensors, biomedical implants, and other materials based on immobilized proteins greatly depend on the method employed to couple the protein molecules to their solid support. Covalent, site-specific immobilization strategies are robust and can provide the | Immobilized Proteins, Protein Chemistry and Applied Microbiology | ResearchGate, the professional network for scientists.

Figure 1 from Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells

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PDF) Recent advances in covalent, site-specific protein immobilization

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A) A protein of interest fused to an enzyme tag specifically binds to

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Affinity Labels for Protein Purification

Sanne SCHOFFELEN, Senior Researcher